Characterization of the cellular and transcriptomic landscapes of natural killer cells in hematopoietic microenvironment of myelodysplastic syndrome

Figure 4 The gene expression pattern of expanded myelodysplastic syndrome natural killers and expanded healthy donor natural killers. A and B: Box plot (A) and RPKM (short for Reads per Kilobase per Million mapped reads) diagram (B) showed the distribution of genes expression pattern in expanded myelodysplastic syndrome natural killers [myelodysplastic syndrome natural killers-1 (MDS-NKs-1), MDS-NK-2, MDS-NK-3] and expanded healthy donor natural killers [healthy donor natural killers-1 (HD-NK-1), HD-NK-2, HD-NK-3]; C and D: Principal components analysis (short for principal component analysis) and Pearson correlation analysis (D) of the indicated NK cells; E and F: Volcano plot diagram (E) and bar chart (F) showed the down-regulated differentially expressed genes (DEGs) (down), the up-regulated DEGs (up), and the non DEGs (no-DEG) between expanded myelodysplastic syndrome natural killers and expanded HD-NKs; G: HeatMap diagram revealed the hierarchical cluster pattern of DEGs between expanded myelodysplastic syndrome natural killers and expanded HD-NKs. MDS-NKs: Myelodysplastic syndrome natural killers; HD-NKs: Healthy donor natural killers; DEG: Differentially expressed genes.