Characterization of the cellular and transcriptomic landscapes of natural killer cells in hematopoietic microenvironment of myelodysplastic syndrome

Figure 3 Cellular viability and cytotoxicity of expanded myelodysplastic syndrome natural killers and expanded healthy donor natural killers upon tumor cell lines. A and B: Representative flow cytometry diagrams (A) and statistical analyses (B) of the apoptotic subsets in expanded myelodysplastic syndrome natural killers (eMDS-NKs) and expanded healthy donor natural killers (eHD-NKs); C and D: Representative flow cytometry diagrams (C) and statistical analyses (D) of cell cycle-related sub-stage distribution (G0/G1, S, G2/M) eMDS-NKs and eHD-NKs; E and F: Representative diagrams (E) and statistical analyses (F) of CD107a⁺ natural killer cells in the indicated groups (Ctr, K562, Nalm6, U937) at an effector-to-target ratio (1:5); G and H: The ex vivo cytotoxicity of eMDS-NKs and eHD-NKs according to the BV421-based fluorescence of the remaining surviving tumor cells. Data were shown as mean ± SEM (n = 3 repetitions for each sample). ^bP < 0.01, NS, not significant. eMDS-NKs: Expanded myelodysplastic syndrome natural killers; eHD-NKs: Expanded healthy donor natural killers.