Characterization of the cellular and transcriptomic landscapes of natural killer cells in hematopoietic microenvironment of myelodysplastic syndrome

Figure 1 Comparison of the immunophenotype and components between resident myelodysplastic syndrome natural killers and resident healthy donor natural killers. A and B: Representative flow cytometry diagrams (A) and statistical analysis (B) of CD3^-CD56⁺ total natural killers (NK) cells in the indicated groups [resident myelodysplastic syndrome NKs (rMDS-NKs), resident healthy donor NKs (rHD-NKs)]; C and D: Representative flow cytometry diagrams (C) and statistical analyses (D) of total activated CD3^-CD56⁺CD16⁺ NK cells and the indicated subsets (NKG2D⁺, CD25⁺, NKp44⁺, and NKp46⁺) in the indicated groups (rMDS-NKs, rHD-NKs); E: Statistical analyses of CD3⁺ total T lymphocytes, CD3⁺CD56⁺ NKT cells, and the T cell subsets (CD3⁺CD4⁺, CD3⁺CD8⁺, and CD4⁺FoxP3⁺ T cells) in the indicated groups (rMDS-NKs, rHD-NKs). Data were shown as mean ± SEM (n = 3 repetitions for each sample). ^aP < 0.05, ^bP < 0.01, NS: Not significant. rMDS-NKs: Resident myelodysplastic syndrome natural killers; rHD-NKs: Resident healthy donor natural killers; NK: Natural killer.