Integrated single-cell transcriptomic analysis of brain and lymph node metastases in non-small cell lung cancer

Figure 5 Myeloid cell landscape in brain and lymph node metastases of non-small cell lung cancer. A: UMAP visualization of 15916 myeloid lineage cells, including macrophages, monocytes, dendritic cells (DC), and neutrophils, derived from non-small cell lung cancer (NSCLC) primary tumors, normal tissues, and metastatic lesions [lymph node metastases (mLN) and brain metastases (mBrain)]; B: UMAP visualization of macrophages, monocytes, DCs, and neutrophils across the five tissue groups; C: UMAP plot of myeloid cell subclusters (generated by subclustering of all myeloid cells), identifying multiple macrophage, monocyte, DC, and neutrophil subsets; D: UMAP plot showing the cell distribution of the 14 myeloid cell subclusters across the five tissue groups; E: Dot plot showing the expression levels of selected canonical marker genes used for cell type identification of the 14 myeloid cell subclusters; F: Stacked bar plot showing the proportion of each cell subset among the five tissue groups; G: Representative graph showing the proportional distribution of myeloid cell subclusters across different tissue samples. Adjusted P values for between-group comparisons are shown at the top; H-I: Dot plot showing the expression levels of key immunosuppressive in myeloid cells of metastatic sites (mBrain and mLN) compared to normal tissues. nLN: Normal lymph nodes; mLN: Lymph node metastases; mBrain: Brain metastases; nBrain: Normal brain tissues.