Integrated single-cell transcriptomic analysis of brain and lymph node metastases in non-small cell lung cancer

Figure 4 T cell landscape in lymph node and brain metastases of non-small cell lung cancer. A: UMAP visualization of 59450 T cells extracted from non-small cell lung cancer (NSCLC) primary tumors, normal lymph nodes (nLN), lymph node metastases (mLN), normal brain tissues, and brain metastases (mBrain). Major immune lineages are annotated, including CD4⁺ T cells, CD8⁺ T cells, and γδT cells; B: Distribution of CD4⁺ T cells, CD8⁺ T cells, and γδT cells across the five tissue groups; C: UMAP plot of T cell subclusters (generated by subclustering of all T cells), identifying multiple CD4⁺ and CD8⁺ subsets as well as γδT cells; D: UMAP plot showing the cell distribution of the 11 T cell subclusters across the five tissue groups; E: Dot plot showing the expression levels of selected canonical marker genes used for cell type identification of the 11 T cell subclusters; F: Stacked bar plot showing the proportion of each cell subset among the five tissue sample groups; G: Representative graph showing the proportional distribution of T cell subclusters across different tissue samples. Adjusted P values for between-group comparisons are shown at the top; H and I: Dot plot showing the elevated expression of exhaustion markers, including PD-1 (PDCD1), TIM-3 (HAVCR2), CTLA4, and LAG3, T cells of metastatic sites (mBrain and mLN) compared to normal tissues. nLN: Normal lymph nodes; mLN: Lymph node metastases; mBrain: Brain metastases; nBrain: Normal brain tissues.