Integrated single-cell transcriptomic analysis of brain and lymph node metastases in non-small cell lung cancer

Figure 2 Different distribution of tumor epithelial subclusters in lymph node and brain metastases of non-small cell lung cancer. A: UMAP visualization of two epithelial subclusters and 14 tumor subclusters identified by subclustering analysis of 26587 epithelial/tumor cells derived from non-small cell lung cancer primary tumors (lung cancer), lymph node metastases (mLN), normal lymph nodes (nLN), brain metastases (mBrain), and normal brain tissues (nBrain). Each subcluster is annotated by its top marker genes; B: The UMAP shows the cell distribution of two epithelial subpopulations and 14 tumor subpopulations in the five tissue groups; C: Dot plot showing the expression levels of selected canonical marker genes used for cell type identification of the two epithelial subclusters and 14 tumor subclusters; D: Stacked bar plot showing the proportion of each tumor subcluster within different tissue types; E: UMAP plot showing the cell distribution of tumor and epithelial tissues in the five tissue groups; F: UMAP plot colored by tumor and epithelial tissues; G: UMAP plot colored by tissue origin (nLN, nBrain, Lung Cancer, mBrain, and mLN). nLN: Normal lymph nodes; mLN: Lymph node metastases; mBrain: Brain metastases; nBrain: Normal brain tissues.