Integrated single-cell transcriptomic analysis of brain and lymph node metastases in non-small cell lung cancer

Figure 1 Single-cell transcriptomic atlas of primary and metastatic non-small cell lung cancer. A: UMAP visualization of 174643 cells integrated from adult human non-small cell lung cancer primary tumors (lung cancer), lymph node metastases (mLN), normal lymph nodes (nLN), brain metastases (mBrain), and normal brain tissues (nBrain). Cells are color coded by annotated cell types: T cells, B cells, plasma cells, myeloid cells, mast cells, microglia, epithelial cells, endothelial cells, myofibroblasts (Myo_fibro), neuroendocrine cells, oligodendrocytes, and smooth muscle cells; B: Dot plot showing expression levels of selected canonical marker genes used for celltype identification; C: UMAP plot colored by tissue origin (nLN, nBrain, Lung Cancer, mBrain, and mLN); D: UMAP plot showing cell type distribution across the five tissue groups; E: Stacked bar plot showing cell proportion per tissue group; F and G: Bar plots highlighting changes in cell type composition between nBrain and mBrain (up) and between nLN and mLN (down). nLN: Normal lymph nodes; mLN: Lymph node metastases; mBrain: Brain metastases; nBrain: Normal brain tissues; ECs: Epithelial cells; SMC: Smooth muscle cells.