GPR81 nuclear transportation is critical for cancer growth and progression in lung and other solid cancers

Figure 2 Nuclear GPR81 is present in non-small cell lung cancer cells and in non-small cell lung cancer tissue sections. Immunohistochemistry (IHC) stain was performed on human lung tissue. A and B: Control and lung cancer. Tissue sections were used in IHC immunostain. IHC was performed using antibodies to GPR81 to display GPR81 distribution in control and cancer tissue sections (red); Counterstain was Mayer’s hematoxylin to display the nucleus (blue). Scale bar = 100 µm; C: GPR81 stain was quantified with Image J and summarized in figure C. Twelve each of control and non-small cell lung cancer (NSCLC) patient specimens, 5 sections were imaged in each specimen; D: Immunoflurescent stain was performed on human NSCLC and control cells with GPR81 antibody. Scale bar = 20 μmol/L; E: GPR81 stain in figure D was quantified with Image J and summarized (4 cell lines each, 202, 205, 272, 392, A549, H661, H858, H645); F: Tissue sections were used in IHC immunostain. IHC was performed using antibodies to GPR81 to display GPR81 distribution in control and cancer tissue section (red); left panels are example images for non cancer liver, prostate, kidney and breast tissue; the middle two panels are example images for liver cancer, prostate cancer, kidney cancer and breast cancer (scale bar = 100 μmol/L). The right panel is a summary of the IHC stain for those cancers. Counterstain was Mayer’s hematoxylin to display the nucleus (blue). Scale bar = 50 µm.