Functional heterogeneity and clinical implications of CD4+ T cell subtypes in high-grade serous ovarian carcinoma

Figure 1 Distinct CD4+ T cell subtypes and functional signatures in high-grade serous ovarian carcinoma microenvironments. A: UMAP visualization showing the distribution of all cell subtypes across samples from 42 high-grade serous ovarian carcinoma patients; B: UMAP visualization demonstrating the anatomical origins of all cells, including samples from ascites and multiple tumor sites; C: UMAP visualization demonstrating distinct transcriptional profiles of CD4+ T cells isolated from adnexal tissues; D: UMAP visualization showing transcriptional profiles of CD4+ T cells isolated from ascites; E: Identification of major CD4+ T cell subtypes: Naive CD4+ T cells, regulatory T cells (Tregs), and CXCL13-expressing CD4+ T cells; F: Distribution of cell counts across the three identified CD4+ T cell subtypes; G: Top-ranked marker genes for each CD4+ T cell subtype identified by differential expression analysis; H: Expression heatmap highlighting key marker genes for distinct CD4+ T cell subtypes, including naive T cells, regulatory T cells (Tregs), and CXCL13-expressing CD4+ T cells. The heatmap reveals subtype-specific transcriptional profiles; I: UMAP feature plots displaying the expression patterns of marker genes for distinct CD4+ T cell subtypes across all sampled tissues; J: Density plots showing the spatial distribution of CD4+ T cells in adnexal tissues (left) and ascites (right) on UMAP projections; K: Pie charts depicting the proportional composition of CD4+ T cell subtypes in adnexal tissues (left) and ascites (right); L: Functional profiling of CD4+ T cell subtypes, comparing signatures for TCR signaling, activation, exhaustion, co-stimulatory molecules, and glycolytic metabolism between adnexa and ascites, highlighting distinct tissue-specific immune profiles.