Yin Yang 1 activates JAK-STAT3-mediated epithelial-mesenchymal transition in Helicobacter pylori-induced gastric cancer progression

Figure 5 Yin Yang 1 promotes epithelial-mesenchymal transition via activation of the Janus kinase 2/signal transducer and activator of transcription 3 pathway. ^aP < 0.001. A: Total and phosphorylated protein levels of Janus kinase (JAK) 2 and signal transducer and activator of transcription 3 (STAT3) in AGS and SGC7901 cells cocultured with Helicobacter pylori were analyzed by Western blotting; B: Expression of JAK2/STAT3 and epithelial-mesenchymal transition (EMT)-related proteins in AGS and SGC7901 cell lines with Yin Yang 1 (YY1) knockdown and overexpression; C: GEPIA database was used to analyze the relationship between YY1 and JAK2 expression in gastric cancer tissues; D: Joint Array for Public Research algorithm predicted the YY1 binding site in JAK2 promoter; E: Interaction between YY1 and JAK3 was detected using chromatin immunoprecipitation assay; F: Activation of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression following addition of coumermycin A1 in AGS cells with YY1 knockdown, and inhibition of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression with addition of AG490 in AGS cells with YY1 overexpression. YY1: Yin Yang 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; JAK: Janus kinase; STAT3: Signal transducer and activator of transcription 3; pJAK2: Phosphorylated Janus kinase 2; pSTAT3: Phosphorylated signal transducer and activator of transcription 3; JASPAR: Joint Array for Public Research; TPM: Transcript per million.