Yin Yang 1 activates JAK-STAT3-mediated epithelial-mesenchymal transition in Helicobacter pylori-induced gastric cancer progression

Figure 3 Helicobacter pylori enhances Yin Yang 1 expression in gastric cancer. ^aP < 0.05, ^cP < 0.001. A: Protein expression of Yin Yang 1 (YY1), N-cadherin, E-cadherin, Snail, and vimentin expression in AGS and SGC7901 cell lines cocultured with Helicobacter pylori (H. pylori) wild type (HPWT) and H. pylori strains ATCC26695 and ATCC43504 by Western blotting; B and C: Western blotting and quantitative real-time polymerase chain reaction were used to measure YY1 expression in H. pylori-negative and -positive tissues; D: Schematic diagram of the TFF1-KO mouse model (gastric cancer) infected with H. pylori; E: Pathological changes in the gastric mucosa of TFF1-KO mice were assessed by hematoxylin and eosin staining, and H. pylori expression was detected by Warthin-Starry staining; F: Immunohistochemical analysis of YY1 and N-cadherin expression in gastric precancerous lesions and gastric cancer tissues in mice. YY1: Yin Yang 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; N(HP-): Normal tissue without Helicobacter pylori infection; N(HP+): Normal tissue with Helicobacter pylori infection; T(HP-): Tumor tissue without Helicobacter pylori infection; T(HP+): Tumor tissue with Helicobacter pylori infection; HP: Helicobacter pylori.