Yin Yang 1 activates JAK-STAT3-mediated epithelial-mesenchymal transition in Helicobacter pylori-induced gastric cancer progression

Figure 1 Yin Yang 1 is upregulated in gastric cancer tissues and cells. ^bP < 0.01, ^cP < 0.001. A and B: Yin Yang 1 (YY1) expression in tissue samples of gastric cancer was predicted by the GEPIA and starBase websites; C: Kaplan-Meier survival curve of YY1 expression data retrieved from the Kaplan-Meier Plotter database; D and E: YY1 level in gastric cancer tissue samples (n = 6) detected by Western blotting and quantitative real-time polymerase chain reaction; F: Immunohistochemical staining of differential YY1 expression in gastric cancer and paired paracancerous tissues; G: Kaplan-Meier survival analysis comparing survival rates of gastric cancer patients with high and low YY1 expression from our study; H and I: Protein and mRNA expression of YY1 in normal gastric epithelial cell line and gastric cancer cell lines (SGC7901, MGC803, AGS, HGC27, MKN45) was examined by Western blotting and quantitative real-time polymerase chain reaction; J: Immunofluorescence staining was used to assess expression and location of YY1 expression in normal gastric epithelial cell line and gastric cancer cell lines (AGS and SGC7901). STAD: Stomach adenocarcinoma; YY1: Yin Yang 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.