Immune regulation of Chidamide-induced Linc01010 accumulation in breast cancer cell death

Figure 4 Chidamide induced programmed death-1 expression regulated tumor necrosis factor-related apoptosis-inducing ligand secretion in natural killer cells. A and B: Content of programmed death-1 (PD-1) in the cell lysate; C: An impact on the secretion of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in natural killer (NK) cells. First, a co-culture model was adopted that the breast cancer MCF-7 or MDA-MB-231 cells treated with 20 μM Chidamide were cultured with NK-92 cells for 24 hours, 48 hours and 72 hours respectively, after transfecting with Linc01010 specific small interfering RNA (siRNA). We measured the content of PD-1 in the cell lysate with enzyme-linked immunosorbent assay (ELISA) to screen the best time for Chidamide to induce PD-1 expression. Next, ELISA assay was determined whether programmed death-ligand 1 proteins had an impact on the secretion of TRAIL in NK cells. The mean ± SD for three independent experiments and analyzed using Statistical Package for Social Science software. In A and B: 20 μM Chidamide vs Basal at ^aP < 0.05, 20 μM Chidamide and Linc01010 siRNA vs 20 μM Chidamide at ^bP < 0.05. In C: 1000 ng PD-L1 vs 0 ng PD-L1 at ^aP < 0.05, 500 ng PD-L1 vs 0 ng PD-L1 at ^bP <0.05, 100 ng PD-L1 vs 0 ng PD-L1 at ^cP <0.05, 1000 ng PD-1 antibody vs 0 ng PD-L1 at ^dP <0.05. CHid: Chidamide; NK: Natural killer; PD-L1: Programmed death-ligand 1; PD-1: Programmed death-1; TRAIL: Tumor necrosis factor-related apoptosis-inducing ligand.