Immune regulation of Chidamide-induced Linc01010 accumulation in breast cancer cell death

Figure 3 The phosphorylation alteration of key factors in the mitogen-activated protein kinases signaling involved in the Linc01010-mitogen-activated protein kinase kinase 6 interaction. A: Silence Linc01010 performance in MCF-7 cells; B: Linc01010-mitogen-activated protein kinase kinase 6 (MKK6) in MCF-7 cells; C: Silence Linc01010 performance in MDA-MB-231 cells; D: Linc01010-MKK6 in MDA-MB-231 cells. After synthesizing and transfecting three oligonucleotides based on the sequence of Linc01010, we screened the small interfering RNA (siRNA) molecules to effectively silence Linc01010 performance in MCF-7 and MDA-MB-231 cells. Western blot was examined the alterations in key factors of the signal transduction pathway during the interaction of Linc01010-MKK6 in MCF-7 and MDA-MB-231 cells. The mean ± SD for three independent experiments and analyzed using Statistical Package for Social Science software. In A and C: Specific siRNA vs control siRNA at ^aP < 0.05, 20 μM Chidamide and specific siRNA vs 20 μM Chidamide at ^bP < 0.05, 20 μM Chidamide and p38 inhibitor vs 20 μM Chidamide at ^cP < 0.05. In B and D: 20 μM Chidamide vs Basal at ^aP < 0.05, 20 μM Chidamide and Linc01010 siRNA vs 20 μM Chidamide at ^bP < 0.05, 20 μM Chidamide and p38 inhibitor vs 20 μM Chidamide at ^cP < 0.05. CHid: Chidamide; MKK6: Mitogen-activated protein kinase kinase 6; PD-L: Programmed death-ligand; siRNA: Small interfering RNA.