Immune regulation of Chidamide-induced Linc01010 accumulation in breast cancer cell death

Figure 2 Linc01010 exercised its regulatory goal by targeting binding to mitogen-activated protein kinase kinase 6 protein through specific exon domains. A and B: Quantitative polymerase chain reaction and luciferase assay for MCF-7 cells were conducted to reveal the functional localization of Linc01010 exon components in breast cancer cells; C and D: Electrophoretic mobility shift assays and RNA-Protein Pull-Down Assay were utilized to investigate evidence of Linc01010 binding to target protein in breast cancer cells. The mean ± SD for three independent experiments and analyzed using Statistical Package for Social Science software, 20 μM Chidamide vs Basal at ^aP < 0.05. CHid: Chidamide; MKK: Mitogen-activated protein kinase kinase.