Abnormal expression and potential clinical value of oncogenic Krüppel-like factor-5 in lung squamous cell carcinoma

Figure 4 Interfering with KLF5 transcription affected xenograft tumor growth. A: Nude mouse xenograft tumor growth after subcutaneous injection of A549 cells transfected with or without Krüppel-like factor 5 intervention (KLF5i) plasmids; B: Xenograft tumor weight (g); C: Xenograft tumor volume; D: Krüppel-like factor 5 (KLF5) straining by IHC in the N-KLF5 group (n = 6); E: KLF5 straining by IHC in the KLF5i group (n = 6); F: KLF5 straining by MIF in the N-KLF5 group; G: KLF5 straining by MIF in the KLF5i group; H: KLF5 in xenograft tumors by Western blotting (top) and their ratios from KLF5i to GAPDH (bottom). ^cP < 0.001, compared with the blank or N-KLF5 group. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; IHC: Immunohisto- chemistry; KLF5: Krüppel-like factor 5; MIF: Multiplex immunofluorescence.