Abnormal expression and potential clinical value of oncogenic Krüppel-like factor-5 in lung squamous cell carcinoma

Figure 3 Intervening KLF5 affected the biological behaviors of lung cancer cells. A: Lung Beas-2B cells and lung cancer SPC-A-1, NCI-1650, A549 and NCI-1975 cells; B: Krüppel-like factor 5 (KLF5) at the protein level in different cells, as determined by Western blotting; C: Ratios of KLF5 to glyceraldehyde-3-phosphate dehydrogenase; D: KLF5 at the mRNA level in different cells, as determined by quantitative real-time PCR (qRT-PCR); E: Proliferating curve of A549 cells divided into blank, N-KLF5 or KLF5 intervention (KLF5i) groups and transfected with KLF5i plasmids; F: Alterations in KLF5 mRNA by qRT-PCR; G: Migrating analysis of A549 cells among different groups; H: Cell cycle alterations. GAPDH: Glyceraldehyde-3- phosphate dehydrogenase. ^aP < 0.05 and ^cP < 0.001, compared with the blank or N-KLF5 group. KLF5: Krüppel-like factor 5.