Update on vascular endothelial Ca2+ signalling: A tale of ion channels, pumps and transporters

doi:10.4331/wjbc.v3.i7.127

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Jul 26, 2012 (publication date) through May 20, 2024

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World Journal of Biological Chemistry

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1949-8454

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Biol Chem. Jul 26, 2012; 3(7): 127-158
Published online Jul 26, 2012. doi: 10.4331/wjbc.v3.i7.127

Table 1 Pattern of expression of InsP3Rs, RyRs and TPCs in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, and immunoprecipitation

	InsP3R1	InsP3R 2	InsP3R 3	RyR1	RyR2	RyR3	TPCs1-2
Human brain microvascular ECs[113]	+(RT-PCR, WB, IC)
Human umbilical vein ECs[106]	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Human microvascular dermal ECs[213]			+(IP)
Human mesenteric artery ECs[127]	+(RT-PCR)			-(RT-PCR)	-(RT-PCR)	+(RT-PCR)
EA.hy926 cells[51,127]	+(RT-PCR)			-(RT-PCR)	-(RT-PCR)	+(RT-PCR)	+(WB)
Mouse cremasteric ECs[108]	+(WB, IC)	+(WB, IC)	+(WB, IC)
Rat hippocampus ECs[413]	-(IHC)	+(IHC)	-(IHC)
Rat aortic ECs[106,107]	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)
Rat mesenteric artery ECs[107]	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)
Rat basilar artery ECs[107]	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)
Rat adrenal medulla microvascular ECs[106]	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Rat splenic sinus ECs[35]				-(IC)	-(IC)	+(IC)
Bovine aortic ECs[104,109]	+(WB, IC)	+(WB, IC)	+(WB, IC)
Ovine uterine artery ECs[414]		+(IP)

RT-PCR: Reverse transcription/polymerase chain reaction; WB: Western blotting; IC: Immunostaining for cultured cells; IHC: Immunohistochemistry; ISH: In situ hybridization; IP: Immunoprecipitation; ECs: Endothelial cells.

Table 2 Pattern of expression of transient receptor potential canonical channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, northern blots, and in situ hybridization

	TRPC1	TRPC2	TRPC3	TRPC4	TRPC5	TRPC6	TRPC7
PCa/PNa and conductance (pS)[202]	Non-selective, 16	2.7, 42	1.6, 60-66	1.1, 30-42	9, 47-66	5, 28-37	0.5-5.4, 25-50
Human pulmonary artery ECs[210,211,219,222,249,261,415,416]	+(RT-PCR, NB, WB, IC)		+/-(RT-PCR)	+(RT-PCR, WB, IC)	-(RT-PCR)	+/-(RT-PCR) +(WB)
Human coronary artery ECs[234,237]	+(RT-PCR, WB, ISH, IHC)		+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH, IHC)	+(RT-PCR, WB, ISH)
Human cerebral artery ECs	+(RT-PCR, ISH, IHC)		+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH, IHC)	+(RT-PCR, ISH)
Human intact mesenteric artery[127]	+(RT-PCR)		+(RT-PCR)	-(RT-PCR)	-(RT-PCR)
Human glomerular ECs						+(WB, IC)
Human preglomerular ECs[244,245]			+(RT-PCR, WB, IHC)			+(IHC)
Human dermal microvascular ECs[18,212,221,222,264]	+(RT-PCR, WB)	-(RT-PCR)	+(RT-PCR)	+(RT-PCR, WB)	-(RT-PCR)	+/-(RT-PCR) +(WB)	+/-(RT-PCR)
HUVECs[127,205,212,215,218,220-223,227,231]	+(RT-PCR, WB)	-(RT-PCR)	+/-(RT-PCR)	+/-(RT-PCR)	+/-(RT-PCR)	+/-(RT-PCR)	+(RT-PCR)
						+(WB)
EA.hy926 cells[127,243,269]	+(RT-PCR)		+/-(RT-PCR) +(WB)	+/-(RT-PCR)	+(RT-PCR, WB)	+/-(RT-PCR)
Mouse aortic ECs[216,228,248,258]	+(RT-PCR, WB, IC)		+(WB)	+(RT-PCR, WB)	+(WB)	+(WB)
Mouse pulmonary artery ECs[218,251,259,262]	+(RT-PCR, WB)	-(RT-PCR)	+/-(RT-PCR)	+(RT-PCR, WB)	-(RT-PCR)	+(RT-PCR, WB)	-(RT-PCR)
Mouse brain capillary ECs	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Bovine pulmonary artery ECs[232]	+(RT-PCR)		-(RT-PCR)	+(RT-PCR)
Bovine aortic ECs[207-209,248,267,268]	+(RT-PCR, WB, IC)		+/-(RT-PCR) +(WB)	+(RT-PCR, WB)	+(RT-PCR, WB)	+(RT-PCR, WB)
Bovine brain capillary ECs	+(RT-PCR)	-(RT-PCR)	+(RT-PCR)	-(RT-PCR)	+(RT-PCR)	-(RT-PCR)	-(RT-PCR)
Bovine adrenal capillary ECs[292]
Rat aortic ECs[233,239]	-(WB)		+(WB)	-(WB)	+(WB)	+(WB)
Rat pulmonary artery ECs[260]	+(RT-PCR)	+(RT-PCR)	+/-(RT-PCR)	+(WB, IC)		-(RT-PCR)	-(RT-PCR)
				-(RT-PCR)
Rat coronary arterioles ECs	+(RT-PCR, IHC)
Rat cerebral artery ECs[311]	+(RT-PCR)
Rat mesenteric artery ECs[227,312]	+(RT-PCR, WB, IHC)		+(WB, IHC)	+(WB, IHC)	+(WB, IHC)
Rat descending vasa recta ECs[254]				+(RT-PCR, WB, IC)	-(RT-PCR)
Rat splenic sinus ECs[35]	+(IHC)
Porcine aortic ECs[241,242]			+(RT-PCR, WB)	+(WB)
Porcine coronary artery ECs[235]			+(RT-PCR, WB)
Zebrafish aortic ECs[225]	+(IHC, ISH)					+(ISH)
Ovine uterine artery ECs[414]	+(WB)		+(WB)	+(WB)	+(WB)	+(WB)

Table 3 Pattern of expression of transient receptor potential vanilloid channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, and in situ hybridization

	TRPV1	TRPV2	TRPV3	TRPV4	TRPV5	TRPV6
PCa/PNa and conductance (pS)[202]	10, 35-80	1-3, NM	12, 172	6, 90	> 100, 75	> 100, 40-70
Human coronary artery ECs[300]				(+RT-PCR, WB, IHC)
Human pulmonary artery ECs[415]	+(RT-PCR)	+(RT-PCR)		+(RT-PCR)
Human pulmonary microvascular ECs	+(RT-PCR)
Human cerebral microvascular ECs[272]	+(RT-PCR, IC)
Human cerebral arterioles ECs[305]			+(RT-PCR, IHC)
Human dermal microvascular ECs[319]				+(RT-PCR, WB)
Breast cancer derived microvascular ECs[319]				+(RT-PCR, WB)
Human umbilical vein ECs[227,277,296,301]	+(RT-PCR)			+(WB, IHC)
Mouse aortic ECs[280,285,299,306]	+(WB)			+(RT-PCR, WB, NM, IHC)
Mouse pulmonary artery ECs[313]				+(WB, IHC)
Mouse mesenteric artery ECs[27,280,302]	+(RT-PCR, WB, IC)			+(RT-PCR, IC)
Mouse cerebral microvascular ECs		+(RT-PCR)		+(RT-PCR, WB)		+(RT-PCR)
Mouse dermal microvascular ECs[307]				+(RT-PCR, WB)
Mouse carotid artery ECs[290,296]				+(IHC)
Rat mesenteric artery ECs[226,275,302]	+(WB)			+(WB, IC, IHC)
Rat femoral artery ECs[312]				+(RT-PCR, IC)
Rat pulmonary artery ECs[303]				+(WB, IHC)
Rat renal artery ECs[303]				+(IHC)
Rat cardiac microvascular ECs[303,316]				+(IHC)
Rat cerebral artery ECs[287,297,311]		+(RT-PCR)	+(RT-PCR, IC)	+(RT-PCR, IC)
Rat carotid artery ECs[291]				+(RT-PCR)
Porcine aortic ECs[312,317]				+(RT-PCR, IC, WB)
Porcine coronary artery ECs[273]	+(IHC)
Bovine aortic ECs[271]	+(WB)
Bovine adrenal capillary ECs[292]				+(RT-PCR, WB)
Zebrafish ventricular ECs				+(ISH)

RT-PCR: Reverse transcription/polymerase chain reaction; WB:Western blotting; IC: Immunostaining for cultured cells; IHC: Immunohistochemistry; ISH: In situ hybridization; NM: Not measured; ECs: Endothelial cells.

Table 4 Pattern of expression of transient receptor potential melastatin channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, Western blotting, and in situ hybridization

	TRPM1	TRPM2	TRPM3	TRPM4	TRPM5	TRPM6	TRPM7	TRPM8
PCa/PNa and conductance (pS)[202]	NM, NM	0.5-1.6, 52-80	0.1-10, 65 (Ca²⁺)-130	Not permeable, 25	Not permeable, 16-25	PCa/PNa~6, NM	3, 40-105	1-3, 83
Human Pulmonary artery ECs[320]	+(RT-PCR)	+/-(RT-PCR) +(WB)	+(RT-PCR)	+(RT-PCR)	-(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Human umbilical vein ECs[325,327,328]				+(RT-PCR, WB)		-(RT-PCR)	+(RT-PCR, WB)
Mouse aortic ECs[329]				+(RT-PCR, WB)			+(RT-PCR)
Human dermal microvascular ECs[327]							+(RT-PCR, WB)
Mouse cerebral microvascular ECs	-(RT-PCR)	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)	-(RT-PCR)	-(RT-PCR)	+(RT-PCR)	-(RT-PCR)
Mouse cardiac microvascular ECs[8]		+(RT-PCR, WB)					+(RT-PCR)
Rat cerebral artery ECs[311]				+(RT-PCR)		+(RT-PCR)	+(RT-PCR)
Rat spinal cord capillary ECs[324]				+(ISH)
Rat mesenteric artery ECs[312]							+(RT-PCR)

RT-PCR: Reverse transcription/polymerase chain reaction; WB: Western blotting; IHC: Immunohistochemistry; ISH: In situ hybridization; NM: Not measured; ECs: Endothelial cells.

Table 5 Pattern of expression of transient receptor potential ankyrin and polycystic channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunohistochemistry, and Western blotting

	TRPA1	TRPP1	TRPP2
PCa/PNa and conductance (pS)[202]	0.8-1.4, NM	1-5, 40-177	4, 137
Human renal artery ECs[332]			+(RT-PCR, WB, IC)
Human umbilical vein ECs[332]			+(RT-PCR, WB)
Mouse aortic ECs[332,333]		+(RT-PCR, WB, IC)	+(RT-PCR, WB)
Mouse femoral artery ECs[332]			+(RT-PCR, WB)
Rat cerebral arteries[287,304,311]	+(RT-PCR, IC)		+(RT-PCR)

RT-PCR: Reverse transcription/polymerase chain reaction; WB: Western blotting; IC: Immunostaining for cultured cells; NM: Not measured; ECs: Endothelial cells.

Citation: Moccia F, Berra-Romani R, Tanzi F. Update on vascular endothelial Ca²⁺ signalling: A tale of ion channels, pumps and transporters. World J Biol Chem 2012; 3(7): 127-158
URL: https://www.wjgnet.com/1949-8454/full/v3/i7/127.htm
DOI: https://dx.doi.org/10.4331/wjbc.v3.i7.127