Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ

Figure 4 Phorbol 12-myristate 13-acetate stimulation of cells co-expressing Ankrd54 and Lyn promotes their co-immunoprecipitation and co-locations. A: Immunoblot analysis of Ankrd54 immunoprecipitates from cells transfected with eGFP-Ankrd54 and/or Lyn with or without PMA stimulation (200 mmol/L, 1 h). Immunoprecipitates and cell lysates were immunoblotted using anti-Ankrd54, anti-Lyn (sc-15, sc-7274), anti-pY-4G10, and anti-β-actin antibodies, ^bP < 0.01; B: Localization analysis of eGFP-tagged Ankrd54 (green) and Lyn (sc-15, alexaFlour546) by immunofluorescence of HEK293 cells with and without PMA treatment (200 nmol/L, 1 h). Delineation of the nucleus by Hoechst staining (blue). Scale bar = 10 μm; PMA: Phorbol 12-myristate 13-acetate.