Arctic ground squirrel neuronal progenitor cells resist oxygen and glucose deprivation-induced death

Figure 5 Arctic ground squirrel neuronal progenitor cell cultured for 21 d in vitro are more vulnerable to oxygen deprivation than cells cultured for 14 d in vitro. Longer time in culture lowers resistance to oxygen deprivation /reoxygenation indicated by an increase in LDH (A) not seen after 14 d in vitro (DIV). However, after 24 h of O₂ deprivation, followed by 24 h of reoxygenation the proportion of neuronal to nonneuronal progenitors did not change (B) while proliferation of NPCs increased (C). Data are from AGS NPCs cultured for 21 DIV that were deprived of oxygen followed by 24 h of reoxygenation. ^aP < 0.05 compared to control, t-test. LDH: Lactate dehydrogenase; NPCs: Neuronal progenitor cells; AGS: Arctic ground squirrel.