Effect of paricalcitol and enalapril on renal inflammation/oxidative stress in atherosclerosis

Figure 5 Western blot analysis and densitometry of protein band analysis to paricalcitol and enalapril alone and in combination for 16-wk on renal inflammatory proteins MCP-1, TNF-α and TGF-β1 expression in ApoE-deficient atherosclerotic mice. A: Western blot analysis of the effect of paricalcitol and enalapril alone and in combination for 16-wk on renal inflammatory proteins MCP-1, TNF-α and TGF-β1 expression in ApoE-deficient atherosclerotic mice; B: Densitometry of protein band analysis show that renal MCP-1, TNF-α and TGF-β1 expression was significantly enhanced (P < 0.01, P < 0.01 and P < 0.001) in ApoE-deficient atherosclerotic mice (n = 3) compared to controls (n = 3). Pricalcitol (n = 3) and enalapril (n = 3) alone and in combination of the two (n = 3) significantly (P < 0.05 and P < 0.001) exerted anti-inflammatory response by down-regulating the MCP-1, TNF-α and COX-2 expression in atherosclerotic mice. The data represent mean ± SE of three independent Western blot experiments.