Apoptosis induced by Fas signaling does not alter hepatic hepcidin expression

Figure 1 Caspase-3 activity and human hepcidin gene (HAMP) expression: HepG2 cells were treated with 50, 100, 150 and 500 ng/mL of CH11 antibody or solvent (control) for 12 h. A: Induction of apoptosis was confirmed by measuring caspase-3 activity, as described in the ‘Materials and Methods’ section. Caspase-3 activity was expressed as nanomole of flurogenic substrate cleaved per milligram of cell lysate protein; B: cDNA, synthesized from RNA isolated from CH11-treated, tunicamycin-treated and respective solvent-treated cells, was employed as a template in Taqman qPCR assays to determine HAMP mRNA expression, as described in Methods. HAMP expression in CH11-treated cells was expressed as fold expression of that in control cells.