High-density lipoprotein endocytosis in endothelial cells

Figure 1 Detailed analysis of high density lipoprotein uptake and high density lipoprotein -derived lipid transfer in human umbilical vein endothelial cells and human coronary artery endothelial cells. A: HUVECs; B: HCAECs. HUVECs and HCAECs were incubated for the indicated time with HDL-Alexa 586, which was additionally labeled with either Bodipy-cholesterol (BP-C; upper panel) or Bodipy-cholesteryl oleate (BP-CE; lower panel). Samples were fixed and imaged using confocal microscopy. Note that brightness and contrast were increased at the 15 min time point of the BP-CE panel for better visibility (lower panel). HUVECs: Human umbilical vein endothelial cells; HCAECs: Human coronary artery endothelial cells; HDL: High density lipoprotein.