Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression

Figure 4 Effect of alcohol on superoxide levels. Hepatocytes, isolated by perfusion from the livers of littermate control (LMC) and Sod2^+/- mice fed with 10% ethanol or plain water (H₂O), were subjected to electron paramagnetic resonance (EPR) spectroscopy, as described in Methods. A-D: Representative EPR spectra; E: Summary of data presented as mean ± SD, n = 6. The letter a indicates statistical significance (^aP < 0.05).