Lipidomic mass spectrometry and its application in neuroscience

Table 3 Class specific scanning parameters of different lipid classes using spatial resolution triple quadrupole mass spectrometer¹

Lipid class	Class-specific pre screen				Second-step quantification
	Ion mode	Daughter ion mass (m/z)	Collision energy (V)	Scan type	Daughter ion mass (m/z)	Collision energy (V)	Scan type
PC	+	184	35	PIS	189, 183.1, 59	35, 35, 24	NLS
lysoPC	+	59	22	NLS	205, 59	34, 22	NLS
PE, lysoPE	-	196	50	PIS	222.2	30	NLS
PI, LysoPI	-	241.1	45	PIS	241.1	45	PIS
PS, lysoPS	-	87.1	24	NLS	87.1	24	NLS
PG, lysoPG	-	153.1	35	PIS	153.1	35	PIS
PA, lysoPA	-	153.1	35	PIS	153.1	35	PIS
CL, monolysoCL	-	Full MS at high resolution
Triglycerides	+
Spingomyelin	+	213.2	50	NLS	213.2	50	NLS
Ceramide	-	256.2	32	NLS			NLS
Sphingoid base	+	48	18	NLS	48	18	NLS
Psychosine	+	180	24	NLS	180	24	NLS
Acyl -carnitine	+	85.1	30	PIS	85.1	30	PIS
Acyl-CoA	-	134	30	PIS	134	30	PIS

¹The parameters have been adopted from a review of vast literature, which has been reviewed in Yang et al[24]. Some lipid extraction for analyses in positive ion mode performed in presence of Sodium or Lithium ion will change the mass to charge ratio (m/z) of ions. Similarly, extraction performed for analyses in negative ion mode in presence of ammonium acetate may change the m/z of ions. MS: Mass spectrometry; PIS: Precursor ion scan; NLS: Neutral loss scan; PC: Phosphocholine; lysoPC: Lysophosphocholine; PE: Phosphoethanolamine; PI: Phosphoinositol; PS: Phosphoserine; PG: Glycerophosphoglycerols; PA: Phosphatidic acid; CL: Cardiolipin.