Update on vascular endothelial Ca2+ signalling: A tale of ion channels, pumps and transporters

Table 1 Pattern of expression of InsP3Rs, RyRs and TPCs in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, and immunoprecipitation

	InsP3R1	InsP3R 2	InsP3R 3	RyR1	RyR2	RyR3	TPCs1-2
Human brain microvascular ECs[113]	+(RT-PCR, WB, IC)
Human umbilical vein ECs[106]	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Human microvascular dermal ECs[213]			+(IP)
Human mesenteric artery ECs[127]	+(RT-PCR)			-(RT-PCR)	-(RT-PCR)	+(RT-PCR)
EA.hy926 cells[51,127]	+(RT-PCR)			-(RT-PCR)	-(RT-PCR)	+(RT-PCR)	+(WB)
Mouse cremasteric ECs[108]	+(WB, IC)	+(WB, IC)	+(WB, IC)
Rat hippocampus ECs[413]	-(IHC)	+(IHC)	-(IHC)
Rat aortic ECs[106,107]	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)
Rat mesenteric artery ECs[107]	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)
Rat basilar artery ECs[107]	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)	+(RT-PCR, IC, IHC)
Rat adrenal medulla microvascular ECs[106]	+(RT-PCR)	+(RT-PCR)	+(RT-PCR)
Rat splenic sinus ECs[35]				-(IC)	-(IC)	+(IC)
Bovine aortic ECs[104,109]	+(WB, IC)	+(WB, IC)	+(WB, IC)
Ovine uterine artery ECs[414]		+(IP)

RT-PCR: Reverse transcription/polymerase chain reaction; WB: Western blotting; IC: Immunostaining for cultured cells; IHC: Immunohistochemistry; ISH: In situ hybridization; IP: Immunoprecipitation; ECs: Endothelial cells.