Septin 8 is an interaction partner and in vitro substrate of MK5

Figure 5 Mitogen-activated protein kinase-activated protein kinase 5 and SEPT8 colocalize with synaptophysin. A: SK-N-DZ cells were transfected with expression vector encoding AsRed-SEPT8 and after 24 h, cells were fixed. Synaptophysin was visualized by staining with anti-synaptophysin fluorescein isothiocyanate (FITC) conjugate antibody (left panels), while AsRed-SEPT8 was visualized directly (red channel; middle panels). A merged image of red and green channels is shown in the right panel; B: SK-N-DZ cells were fixed and stained with anti-synaptophysin FITC conjugate antibody (left panel) and with anti-PRAK antibody followed by Alexa Fluor 568 anti-rabbit antibody (middle panel). Merged image of red and green channels is shown in the right panel.