High tolerance to mutations in a Chlamydia trachomatis peptide deformylase loop

Figure 6 Purified recombinant chlamydial peptide deformylase proteins. Chlamydial peptide deformylases carrying a (His)₆-tag were expressed in Escherichia coli, purified by cobalt agarose, resolved by 13.5% SDS-polyacrylamide gel, and visualized by Coomassie blue staining. In the left lane are protein standards with molecular weights indicated.