Recent perspectives into biochemistry of decavanadate

Figure 4 F-actin cysteine redox state, after 20 min exposure to decavanadate. Titration of cysteine was performed with 0.1 mmol/L 5,5’-ditiobis-(2-nitrobenzoic acid) and 2 μmol/L actin in 2 mmol/L Tris (pH 7.5), and 0.2 mmol/L CaCl₂. The increase in absorbance at 412 nm was continuously recorded over 10 min; To measure total cysteines the samples were treated afterwards with 1% SDS, and the absorbance was measured, over 15-30 min, until a steady value was reached. Titration with decavanadate produced a dose-dependent decrease in F-actin total cysteines, while Cys-374 (also named "fast cysteine") is reduced. The results shown are the average of triplicate experiments.