Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods

Figure 1 Schematic overview of the RNA presentation and quality assessment workflow for human pancreatic tissue biopsies. Generated using BioRender and manually refined by the authors. Donor pancreata (n = 4) were dissected into head, body and tail regions with three biopsies per region allocated to four preservation methods: Immediate RNA isolation, snap freezing in an isopentane-dry ice slurry, submersion in RNAlater, or injection with RNAlater (144 biopsies). Both RNAlater conditions were incubated at room temperature for 15 minutes before snap freezing and storage at -80 °C. All samples underwent RNA extraction using the RNeasy Mini Kit (Qiagen) with bead-based homogenisation. RNA concentration and purity were assessed by NanoDrop and integrity by Agilent 4200 TapeStation following dilution to 250 ng/μL.