Dithiothreitol induced endoplasmic reticulum stress and its role in neurodegeneration in Caenorhabditis elegans

Figure 6 Effect of dithiothreitol on worms lifespan and survival. A: Dithiothreitol (DTT) treatment reduces worm lifespan in a dose-dependent manner 10 × Microscopic images showing worm survival over days; B: Kaplan–Meier plot reveals significant lifespan reduction, with complete mortality within one day at 20 mmol/L; C: Survival table showing the number of live Caenorhabditis elegans counted daily after transfer to DTT-treated and control plates. On Day 2, approximately 20 synchronized L4-stage worms were transferred to each plate containing 0, 2.5, 5, 10, or 20 mmol/L DTT. The table records the number of surviving worms over 21 days, demonstrating a concentration-dependent decrease in lifespan, with complete mortality observed within 24 hours at 20 mmol/L DTT. DTT: Dithiothreitol.