Dithiothreitol induced endoplasmic reticulum stress and its role in neurodegeneration in Caenorhabditis elegans

Figure 5 Effect of dithiothreitol on growth and reproductive activity in Caenorhabditis elegans. Representative microscopic images showing developmental stages of worms after 5–7 days of exposure to increasing concentrations of dithiothreitol (DTT). Worms on control plates (0 mmol/L DTT) reached L2–L4 larval stages, while 25 mmol/L DTT-treated worms were mostly in L1–L2 stages. At five mM DTT, early L1 larvae and embryos were predominant, indicating developmental delay. Exposure to 10 mmol/L DTT resulted in the presence of gravid adults and embryos with minimal larval development, suggesting reproductive and developmental impairment in a dose-dependent manner. DTT: Dithiothreitol.