Dithiothreitol induced endoplasmic reticulum stress and its role in neurodegeneration in Caenorhabditis elegans

Figure 4 Chemotaxis assay. A: Collect data by counting the number of worms on the attractant (test) and the number on the control spots (10 × microscopic images); B: The formula for calculating chemotaxis index; C: Triplicate data of chemotaxis index at different concentrations of dithiothreitol (DTT); D: A significant reduction in chemotaxis was observed at five mM DTT as compared to control (0 mmol/L). Data represent mean ± SEM (n = 3). Statistical significance was determined using Brown-Forsythe and Welch one-way analysis of variance, followed by Dunnett's T3 post hoc test and Kruskal-Wallis test; ^aP < 0.05. DTT: Dithiothreitol.