Dithiothreitol induced endoplasmic reticulum stress and its role in neurodegeneration in Caenorhabditis elegans

Figure 3 Thrashing assay. A: Thrashing count analysis starting from the left: The first, 3^rd, and last image shows the worm in complete thrashing. The second, 4^th, and 5^th frame shows an incomplete movement where the worm does not fully bend to the opposite side; B: Average thrashing count per minute at different dithiothreitol (DTT) concentrations (0-10 mmol/L); C: The thrashing graph shows the decline in count where animals were exposed to increasing concentrations of DTT (0–10 mmol/L). Data are presented as mean ± SEM. Statistical analysis was performed using the Kruskal-Wallis test. ^aP < 0.001 vs control (0 mmol/L DTT). DTT: Dithiothreitol.