Increased monoamine oxidase activity and imidazoline binding sites in insulin-resistant adipocytes from obese Zucker rats

Figure 3 Increased monoamine oxidase and decreased semicarbazide-sensitive amine oxidase protein expression in subcutaneous adipose tissue from obese rats when compared to lean littermates. Proteins solubilized from white adipose tissue homogenates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted proteins detected using primary antibodies directed against monoamine oxidase (MAO) or semicarbazide-sensitive amine oxidase (SSAO). 50 µg and 5 µg proteins per lane were deposed for MAO and SSAO immunoblots, respectively. A: Immunoblots showing immunoreactive MAO-A and MAO-B at approximately 61 and 55 kDa (arrows) for obese (O) and lean (L) rats; B: Red ponceau control of protein loading for a representative membrane shown in panel A; C: Representative immunoblot of SSAO showing a major band at approximately 85 kDa (arrow); D: Densitometry analysis of the major bands is shown for 7-8 rats per genotype. Different from lean (open columns) at: ^aP < 0.05.