Basic Study
Copyright ©The Author(s) 2021.
World J Biol Chem. Jan 27, 2021; 12(1): 1-14
Published online Jan 27, 2021. doi: 10.4331/wjbc.v12.i1.1
Figure 5
Figure 5 Inhibition of cyclic adenosine monophosphate phosphodiesterase inhibits lipopolysaccharide-induced matrix-metalloproteinase-9 and tumor necrosis factor α in a dose-dependent fashion. A: SDS-PAGE/WB of cell supernatants; and B: Densitometry of the matrix-metalloproteinase-9 (MMP-9) immunoblot in Panel A (circles). Each data point is the average ± standard error for three independent densitometry measurements. Tumor necrosis factor α (TNFα) levels (diamonds) were determined in cell supernatants by enzyme-linked immuno sorbent assay and each data point is the average ± standard error for three independent measurements. THP-1 monocytes were treated with a concentration range of 3-isobutyl-1-methylxanthine (0.01-0.3 mmol/L) followed by lipopolysaccharide (1 μg/mL) for 48 h. Cells were collected, centrifuged at 500 g for 10 min and supernatant assessed for (A) MMP-9 and (B) TNFα. MMP-9 and TNFα data points were fit to a nonlinear inhibition equation, which produced IC50 values of 0.05 mmol/L and 0.08 mmol/L for MMP-9 and TNFα inhibition respectively. LPS: Lipopolysaccharide; MMP-9: Matrix-metalloproteinase-9; TNFα: Tumor necrosis factor α; IBMX: 3-Isobutyl-1-methylxanthine.