Coexistence of esophageal verrucous carcinoma in situ and gastric intramucosal poorly differentiated adenocarcinoma: A case report

Figure 5 Immunohistochemical staining features of the esophageal endoscopic submucosal dissection specimen. A: D240 staining shows continuous linear positivity along the membranes of basal cells (clone number: D2–40, positivity was defined as brownish-yellow staining of the basal cell membrane); B: Ki67 staining shows a marked increase in positive cells in the basal layer, with an abnormal distribution pattern [clone number: 176B3C4, abnormal Ki-67 distribution in the squamous epithelium was defined by the presence of any of the following: (1) A higher number of positive cells in the basal layer (≥ 5/HPF); (2) No or significantly reduced expression of cells in the parabasal layer ( < 50%), or an equal or higher number of positive cells in the basal layer compared to the parabasal layer; and (3) The presence of Ki-67 expression in the epithelial superficial layer (> 2 cells/HPF)]; C: P53 staining shows diffuse strong nuclear expression (clone number: 882F5H1, positivity was defined as brownish-yellow staining of the squamous epithelial cell nucleus); D: P63 staining shows positive cytosolic nuclei in tumour cells (clone number: 281B6A9, positivity was defined as brownish-yellow staining of the squamous epithelial cell nucleus).