Optimizing radiofrequency ablation parameters for perianal fistula treatment: A porcine model study

Figure 2 Ex vivo platform for preliminary screening radiofrequency ablation temperature-time settings. A: Schematic of the segmental radiofrequency ablation catheter positioned along the midline of a fresh porcine loin; the loin was then folded over the catheter so that the loin enveloped the electrode and maintained continuous, uniform contact during energy delivery; B: Loin re-opened after ablation (representative condition: 100 °C/28 seconds), showing a well-demarcated linear ablation tract. This configuration enabled direct caliper measurement of ablation depth and length for each candidate settings.