Individual chemotherapy for patients colorectal cancer based on patient-derived tumor-like cell clusters

Figure 4 Diversity and histological characterization of colorectal cancer patient-derived tumor-like cell clusters. A: Patient-derived tumor-like cell clusters (PTCs) showed different growth rates. Colorectal cancer (CRC)-31, CRC-27, and CRC-23 were treated with drugs on day 5, day 2, and day 4, respectively. At the stationary phase, CRC-31 microspheres were 40-60 μm in diameter, CRC-23 microspheres were 50-120 μm in diameter. Scale bar, 100 μm; B: Diameter distribution of 54 PTC models; C: Culture time required and the corresponding cases of PTC models at stationary phase; D: The comparison of PTCs’ morphology through microscopic images and hematoxylin and eosin (HE) staining. HE staining of CRC-46 showed acinus, CRC-14 showed non-dense morphology with poor cell cohesion, CRC-43 showed dense and strong cohesion relatively. Scale bar, 100 μm (up), 20 μm (down). CRC: Colorectal cancer.