Silencing of thrombospondin-1 prevents abdominal adhesion formation by reducing inflammation and oxidative stress

Figure 5 Thrombospondin-1 deficiency is a protective factor in mesothelial cell line. A: The levels of thrombospondin-1 (Thbs-1) protein in the supernatant of the HMrSV5 cell culture medium were measured by enzyme-linked immunosorbent assay. n = 3; B: Quantitative real-time polymerase chain reaction was used to assess Thbs-1 mRNA expression in different groups. n = 5; C: The cell viability of HMrSV5 cells after transforming growth factor-β exposure measured by Cell Counting Kit-8 assay; D: Images of scratch-wound assay in different groups at 0 hour, 12 hours and 24 hours’ time intervals post injury. n = 5, 100 × magnification; E: Cell migration ratio in different groups. n = 5; F: Reduced reactive oxygen species (ROS) levels of HMrSV5 cells treated with H₂O₂ for 1 hour observed by fluorescence microscopy. n = 3, 200 × magnification; G: Quantification of the ROS levels of mesothelial cells observed by fluorescence microscopy. NC: Negative control; Thbs-1: Thrombospondin-1.