Silencing of thrombospondin-1 prevents abdominal adhesion formation by reducing inflammation and oxidative stress

Figure 3 Thrombospondin-1 deficiency can reduce inflammation and reactive oxygen species formation in adhesion tissue (five groups, n = 5 mice/group). A: Typical hematoxylin-eosin staining images of adhesion tissues in different groups after peritoneal injury; B: Representative images showing reactive oxygen species accumulation (red fluorescence), DAPI (blue fluorescence) and merge in adhesion tissues after peritoneal injury; C: Inflammation score in different groups; D: Malondialdehyde levels were evaluated in adhesion tissues; E: Superoxide dismutase levels were evaluated in adhesion tissues; F: Glutathione peroxidase levels were evaluated in adhesion tissues. H&E: Hematoxylin-eosin; NC: Negative control; Thbs-1: Thrombospondin-1; NS: Normal saline; LSKL: Leu-Ser-Lys-Leu; ROS: Reactive oxygen species; MDA: Malondialdehyde; SOD: Superoxide dismutase; GPx: Glutathione peroxidase.