Septic shock reduces intestinal microcirculation and anastomotic perfusion: A porcine laser speckle contrast imaging study

Figure 2 Laser speckle contrast imaging measurements of the anastomose for colon at specific time points. A: Colon hand-sewn; B: Colon stapled. The placement of regions of interest (ROIs) is indicated on all white light images using bold markings: ROIs were placed adjacent to each other, with ROI 1 centered on the anastomosis, ROI 2 ± 5 mm on either side, and ROI 3 ± 10 mm on either side of the anastomosis. A color scale ranging from red to blue indicates good to poor perfusion, respectively. T_A = Immediately after anastomosis formation; T₀ = One hour of rest/start of Escherichia coli (E. coli) infusion; T₆₀, T_120, T₁₅₀ = Measurements taken during E. coli infusion/sepsis, number indicates minutes from start of E. coli infusion.