Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing

Figure 2 Analysis of 16S rRNA gene sequences and bile microbiome diversity. Statistical significance vs the control was defined as a two-tailed ^aP < 0.05. A: Venn on operational taxonomic units level; B: Rank-Abundance curves on operational taxonomic units level; C: Alpha diversity analysis of bile microbiome including Shannon, Chao, Simpson, Ace, Coverage, and Sobs index; D: Beta diversity analysis of bile microbiome. OUT: Operational taxonomic units; GS: Gallstone; GSF: Gallstone-free; PCoA: Principal coordinate analysis; PC1: Principal component 1; PC2: Principal component 2.