Visual screening and efficacy evaluation of high-performance fluorescent probe DAF-FM in esophagitis cancer transformation

Figure 4 Targeted colocalization of DAF-FM probe with lysosomes. A: Merged images of DAF-FM (red) with Lyso-Tracker Green (lysosome, green), Mito-Tracker Green (mitochondria, green), and BODIPY493/593 (liposome, green); B: Bar chart of Pearson colocalization coefficients (lysosome: 0.82 ± 0.03, mitochondria: 0.21 ± 0.04, liposome: 0.18 ± 0.03). Scale bar: 10 μm; data are expressed as mean ± SD, n = 3 per group, ^bP < 0.01 vs mitochondria/Liposome groups.