Visual screening and efficacy evaluation of high-performance fluorescent probe DAF-FM in esophagitis cancer transformation

Figure 2 Concentration-dependent and time-dependent fluorescence intensity of DAF-FM probe with nitric oxide. A: Fluorescence images of DAF-FM incubated with different concentrations of nitric oxide (NO) donor DEANO (0 μM, 50 μM, 150 μM, 300 μM) for 60 minutes; B: Fluorescence intensity-time curve (0–60 minutes) of DAF-FM at different NO concentrations [0 μM: From 52 ± 3 to 55 ± 4 arbitrary units (a.u.); 300 μM: From 223 ± 9 to 458 ± 15 a.u.]; C: Fluorescence intensity-concentration scatter plot of DAF-FM at 60 minutes (R² = 0.98, P < 0.05). Scale bar: 10 μm; data are expressed as mean ± SD, n = 3 per group.