Protective effects of treprostinil and ischemic preconditioning on hepatic ischemia-reperfusion injury and biomarkers in experimental studies in rats

Figure 6 Representative histopathological images of heart tissue in sham group, hepatic ischemia-reperfusion injury group, treprostinil treated group, and ischemic preconditioning group. A: Sham group; the myocardium in a rat from the sham group shows well-preserved cardiomyocytes. Mild interstitial edema, sinusoidal hyperhemia and presence of wavy fibers located subepicardially are also observed. Hematoxylin and eosin (HE), original magnification, 100 ×; B: Hepatic ischemia-reperfusion injury group (HIRI); rat subjected to HIRI shows loss of striation with homogenously eosinophilic sarcoplasm, a large mononuclear cell aggregate and pyknotic nuclei (necrosis). HE, original magnification, 100 ×; C: Treprostinil-treated group; treprostinil-treated rat shows mild edema and cardiomyocyte necrosis. HE, original magnification, 200 ×; D: Ischemic preconditioning (IP) group; rats subjected to hepatic IP show mild interstitial edema, segmental loss of striation and eosinophilic sarcoplasm. HE, original magnification, 400 ×; E: IP group, rats subjected to hepatic IP show hyperhemia, hemorrhage and edema. HE, original magnification, 100 ×; F: IP group; rats subjected to hepatic IP show eosinophilic sarcoplasm, pyknotic nuclei necrosis and presence of sparse mononuclear cells. HE, original magnification, 200 ×.