Protective effects of treprostinil and ischemic preconditioning on hepatic ischemia-reperfusion injury and biomarkers in experimental studies in rats

Figure 2 Effect of treprostinil and ischemic preconditioning in rats subjected to hepatic ischemia-reperfusion injury. A: Effect of treprostinil and ischemic preconditioning (IP) on serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and lactate dehydrogenase (LDH) levels in rats subjected to hepatic ischemia-reperfusion injury (HIRI). Serum SGOT, SGPT, and LDH levels in the HIRI group were significantly different from sham group. In treprostinil and IP groups, serum SGOT, SGPT, and LDH levels were significantly decreased compared to HIRI; B: Effect of treprostinil and IP on serum creatine phosphokinase (CPK) values in rats subjected to HIRI. Serum CPK levels in the HIRI group were significantly different from the sham group, confirming the presence of myocardial injury. In treprostinil and IP groups, serum CPK levels were reduced compared to the HIRI group, but no significant difference was observed; C: Effect of treprostinil and IP on serum troponin I values in rats subjected to HIRI. Serum troponin I levels in the HIRI group were significantly different from the sham group, confirming the presence of cardiac damage. The treprostinil group significantly reduced serum troponin I levels. Rats pre-tretated with IP also had lower serum troponin I levels, but no statistically significant difference was observed; D: Effect of treprostinil and IP on serum tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1) values in rats subjected to HIRI. Serum TNF-α and ET-1 levels in the HIRI group were significantly elevated compared with the sham group, confirming the upregulated inflammatory response. In the treprostinil and IP group, serum TNF-α and ET-1 levels were significantly reduced compared to the HIRI group. Black lines on the top of the bars represent SD. HIRI: Hepatic ischemia-reperfusion injury; IP: Ischemic preconditioning; SGOT: Serum glutamic-oxaloacetic transaminase; SGPT: Serum glutamic pyruvic transaminase; LDH: Lactate dehydrogenase; CPK: Creatine phosphokinase; TNF-α: Tumor necrosis factor-α; ET-1: Endothelin-1. ^aP < 0.001, ^bP < 0.05, and ^cP > 0.05.