ANXA13 expression patterns in hepatocellular carcinoma and impact on tumor behavior

Figure 2 Expression levels of the ANXA13 gene in normal liver cell lines and three types of liver cancer cell lines (Hep3B, Huh7, HepG2). A: Quantitative real-time PCR was used to detect the expression of the ANXA13 gene in the normal liver cell line (WRL) and three hepatocellular carcinoma (HCC) cell lines (Hep3B, Huh7, HepG2); B: Western blot was used to detect the expression levels and band intensities of ANXA13 protein in the WRL and three HCC cell lines (Hep3B, Huh7, HepG2). GAPDH was used as a loading control. Unpaired Student’s t-test and analysis of variance were used for data analysis. All data were representative of at least three independent experiments (n = 3; error bar, SD). ^bP < 0.001; ^cP < 0.0001.