ANXA13 expression patterns in hepatocellular carcinoma and impact on tumor behavior

Figure 1 Expression levels of ANXA13 in hepatocellular carcinoma tissues and adjacent non-tumor tissues. A: Detection of ANXA13 expression in liver cancer tissues and adjacent non-cancerous tissues by quantitative real-time PCR; B: Western blotting shows that the ANXA13 protein is overexpressed in Hepatocellular carcinoma (HCC) tissues. GAPDH was used as a loading control. Use Image J software to analyze protein bands for grayscale analysis, and normalize the grayscale values of the target protein before using GraphPad Prism software for statistical analysis; C: HCC and adjacent non-tumor tissues were subjected to immunohistochemical analysis using Diaminobenzidine staining. Representative images are shown (left panel, Scale bar: 50 μm), and the staining intensity was compared (right panel). Unpaired Student’s t-test was used for data analysis. All data were representative of at least three independent experiments (n = 3; error bar, SD). ^bP < 0.001; ^cP < 0.0001. HCC: Hepatocellular carcinoma.