Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus

Figure 3 Immunocytochemical analysis of peripheral blood leukocytes in rats depending on the content of anti-apoptotic Bcl-2 protein in healthy animals, animals with streptozotocin-induced diabetes and treated with the submerged cultured mycelium powder of mushrooms. A: Control; B: Control animals treated with G. lucidum; С: Animals with STZ-induced DM; D: Diabetic animals treated with G. lucidum. 1: Bcl-2^-; 2: Bcl-2⁺; 3: Bcl-2⁺⁺. An indirect immunoperoxidase method was used for detection and visualization of intracellular proteins Bcl-2. The content analysis of Bcl-2 in leukocytes of rat peripheral blood was performed by light microscopy using a × 40 microscope objective. Depending on intensity of staining, the cells were divided into 3 groups: Negative reaction (Bcl-2^-), positive reaction (Bcl-2⁺), and high-positive (Bcl-2⁺⁺) reaction. G. lucidum: Ganoderma lucidum; STZ: Streptozotocin; DM: Diabetes mellitus.